Key result
Bendroflume-thiazide reduced urinary calcium excretion to 10% and furosemide increased it by 50%, but neither altered renal calbindin-D28k concentrations compared to vehicle.
Why the study?
Does bendroflumethiazide infusion alter renal calbindin-D28k concentrations in Wistar rats?
Does bendroflumethiazide infusion alter renal calbindin-D28k concentrations in Wistar rats?
Urinary calcium excretion can be selectively manipulated without accompanying changes in renal calbindin-D28k concentrations, suggesting it is not a significant determinator of cytosolic calbindin-D28k.
Answers are grounded in this paper and cite their sources.
No support for calbindin-D28k mediation of diuretic calciuric effects in rats; leaves open its role in human calcium disorders.
The present investigation examined the possible influence of urinary calcium excretion on the concentration of renal calbindin-D28k. Thiazide diuretics stimulate calcium transport across the epithelial cells of the distal tubule, which express calbindin-D28k in high concentrations. Calbindin-D28k is assumed to facilitate transcellular Ca diffusion. Reduced urine calcium excretion and increased urine output were induced in Wistar rats by infusion of bendroflume-thiazide 1 mg/kg/day. The two control groups had infusions of either furosemide 20 mg/kg/day or vehicle, n = 8 in each group. Urinary Ca excretion was reduced to 10% in the thiazide group and increased by 50% in the furosemide group. Renal concentrations of calbindin-D28 showed no difference between vehicle, thiazide- and furosemide-treated rats. No differences in plasma concentrations of calcium, magnesium, phosphorus, urea, PTH, calcitonin and 1,25-(OH)2D were found between the groups. The present study describes that urine calcium excretion selectively can be manipulated without accompanying changes in renal calbindin-D28k concentrations. The data, therefore, suggest that urinary calcium excretion is not a significant determinator of cytosolic concentrations of renal calbindin-D28k.
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Hemmingsen et al. (1997) studied this question. bendroflume-thiazide vs. furosemide 20 mg/kg/day or vehicle was evaluated on Renal concentrations of calbindin-D28k. Bendroflume-thiazide reduced urinary calcium excretion to 10% and furosemide increased it by 50%, but neither altered renal calbindin-D28k concentrations compared to vehicle.
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