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July 25, 2025Circulation ResearchOpen Access

Patients with HFpEF exhibited a distinct peripheral blood mononuclear cell transcriptional immune signature characterized by obesity-related cytokine signaling and mitochondrial-associated activity.

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Why the study?

Intramyocardial inflammatory activity in HFpEF myocardial biopsies suggests inflammatory processes are predominantly systemic with compartment-specific patterns, closely related to extracardiac comorbidities.

Population

Patients with HFpEF (n=6), HFrEF (n=8), and healthy controls (n=7), plus an HFpEF mouse model

Comparison

HFpEF vs HFrEF vs healthy controls, and HFpEF mice with vs without nitro-oleic acid

Design

Translational single-cell and bulk RNA sequencing study with a parallel mouse model

Key result

Patients with HFpEF exhibited a distinct peripheral blood mononuclear cell transcriptional immune signature characterized by obesity-related cytokine signaling and mitochondrial-associated activity.

Authors

JKJasmin M. KneuerMMMarion MüllerSEStephan Erbe

Discussion

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Overview

Hypothesis-generating for targeted anti-inflammatory therapies in HFpEF; leaves open whether modulating.

Study Design

Type

Observational (n=21)

Structured PICO

P
Population
21 human subjects (6 with HFpEF, 8 with HFrEF, and 7 healthy controls) evaluated for systemic immune cell transcriptional signatures.
E
Exposure
Nitro-oleic acid (NO2-OA) administered to the HFpEF mouse model.
C
Comparator
Patients with HFrEF and healthy controls (human cohort); untreated HFpEF animals (mouse model).
O
Outcome
Systemic immune cell transcriptional signature (assessed via single-cell RNA sequencing of PBMCs) and diastolic function.surrogate

HFpEF is associated with a distinct, conserved systemic inflammatory transcriptional signature that may serve as a target for personalized interventions such as nitro-oleic acid.

Cite This Study

Kneuer et al. (2025) conducted an observational in Heart failure with preserved ejection fraction (HFpEF) (n=21). Heart failure with preserved ejection fraction (HFpEF) vs. HFrEF and healthy controls was evaluated on Peripheral blood mononuclear cell transcriptional immune signature. Patients with HFpEF exhibited a distinct peripheral blood mononuclear cell transcriptional immune signature characterized by obesity-related cytokine signaling and mitochondrial-associated activity.

synapsesocial.com/papers/6a8f736da7049199b0c7ee05https://doi.org/10.1161/circresaha.125.326249
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Comparative immune profiling in HFpEF: cross-species insights into inflammation and mitochondrial dysfunction2025
  2. 2Normal Abundance of Immune Cells in Left Ventricular Myocardium from Patients with Heart Failure and Preserved Ejection Fraction2026 · 1 citations
  3. 3Single-cell transcriptomics reveal distinctive patterns of fibroblast activation in murine heart failure with preserved ejection fraction2023 · 2 citations
  4. 4Impact of Voluntary Exercise on Systemic Inflammation in a 2? Hit Model of Heart Failure With Preserved Ejection Fraction2025
  5. 5Myocardial Inflammatory Remodeling in HFpEF: Potential Role of Chemoreflex-Induced Low-Grade Inflammation2026