The toxicity of xylene to human health and the environment warrants its careful control or, ideally, its elimination from the laboratory. Although several xylene substitutes are now available, a comprehensive evaluation of them has yet to be published. This study investigated the use of 7 xylene substitutes of 2 classes, limonenes and alkanes, for paraffin tissue processing. Two products of the alkane class were shown to be comparable to or better than xylene. (The J Histotechnol 17:143, 1994)
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Maria Wynnchuk (1994) studied this question.
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