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S ummary : New culture conditions are described for the growth of Clostridium histolyticum allowing for the production of collagenase (E.C.3.4.4.19) in a highly purified form, free of general protease activity. The conditions include the use of a mixture of sodium bisulphite‐oxidized sodium thioglycollate to provide the reducing environment within an otherwise standard culture medium. Also, new modifications in the preparation of collagenase A are reported.
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Takahashi et al. (1972) studied this question.
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