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August 27, 2026Zoo BiologyOpen Access

Fecal Glucocorticoid and Androgen Metabolites in Eastern Gray Kangaroos ( Macropus giganteus ) Following Environmental Relocation

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Authors

LMLaura MenchettiRPRupert PalmeLGLivio Galosi

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Overview

Observational study reveals rapid physiological adaptation via fecal hormone metabolites in eastern gray kangaroos following relocation, indicating their utility for noninvasive welfare monitoring.

Key Points

  • To monitor hypothalamic-pituitary-adrenal axis response and physiological adaptation in eastern gray kangaroos following institutional relocation using noninvasive fecal hormone metabolite analysis.
  • Monitored 3 eastern gray kangaroos (two females, one male; N=3) relocated between two zoological facilities in Italy from January 20 to April 9, 2024.
  • Collected fecal samples daily for 10 days post-arrival and weekly thereafter to quantify glucocorticoid metabolites (GCMs) and androgen metabolites (AMs) via enzyme immunoassays.
  • Fecal GCM levels initially spiked above 100 ng/g (range: 14–262 ng/g) before declining and stabilizing by day 10, with a secondary elevation in mid-March.
  • Fecal AMs decreased from 34 ± 21.5 ng/g in the first week to 9 ± 3.6 ng/g over the subsequent 40 days, displaying a strong positive correlation with GCMs (ρ = 0.741, p < 0.001).
  • The GCM/AM ratio initially exceeded 5 in all subjects before declining rapidly, reflecting acute initial stress followed by physiological acclimatization.

Cite This Study

Menchetti et al. (2026) studied this question.

synapsesocial.com/papers/6a8fe9da10c91c1e92621d8dhttps://doi.org/10.1002/zoo.70096
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