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The intrinsic viscosity of C hordein was determined in 70% ( ) aqueous ethanol (± 10 mM NaCl) and 0.1 M acetic acid at temperatures between 15.3 and 50°C. Calculations based on the assumption (supported by previously published results) that C hordein has an ordered conformation indicated that the molecule is rod shaped, with molecular dimensions varying from about 360 × 17 Å to 265 × 20 Å. Circular dichroism spectroscopy indicated a secondary structure rich in β‐turns, with some conformational differences in the two solvents and at different temperatures. The results are compatible with C hordein having a helical secondary structure based on repetitive β‐turns.
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Field et al. (1986) studied this question.
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