A simple, isocratic high performance liquid chromatography system (HPLC) with electrochemical detection (EC) was used to study Cannabis constituents. Several Cannabis extracts and reference standards were examined. A total of eleven constituents were separated, and three major cannabinoids; Δ8, Δ9 tetrahydrocannabinol (THC) and cannabidiol (CBD) were identified. A linear relationship was established for the quantitation of the halucinogenic constituent Δ9 THC. Minor contaminants in Δ9 THC reference standard, which were not detected by gas chromatography (GC) were detected for the first time. The detection limits of Δ9 THC and related cannabinoids were in the low nanogram range (2–20 ng).
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Masoud et al. (1979) studied this question.
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