Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 3. Methods for the disruption of Krebs II mouse-ascites-tumour cells

We have reported on the changes in nucleic acid and protein metabolism in Krebs II ascites-tumour cells during the growth of encephalomyocarditis virus in this cell (Martin, Malec, Sved & Work, 1961). For further work, it was decided to study the metabolic changes in different subcellular fractions of the Krebs cell. Before this could be done, however, methods had to be devised for the rupture of Krebs cells and for the quantitative separation of the subcellular particles.