Key result
Standardized methods for the extraction and concentration of enteric viruses from fresh produce are needed, as current methodologies lack validated consensus protocols.
The review highlights the critical need for sensitive, reliable, and internationally standardized methods for the extraction and concentration of enteric viruses from fresh produce to improve food safety monitoring.
May compromise produce-related outbreak detection; leaves open validation of international consensus protocols.
Virus-contaminated soft fruits or vegetables are increasingly identified as causes of foodborne viral illness. Noroviruses and hepatitis A virus are the most common pathogens in viral infections transmitted by these kinds of foods. To improve microbiological detection and monitoring and to increase insights into the contribution of fruits and vegetables to foodborne viral transmission, sensitive, reliable, and standardized methods are needed. More studies on virus detection methods for foods are being published, but validated consensus protocols are not yet available. In this paper, different methodologies are reviewed critically. The use of process controls and internal amplification controls is discussed.
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Croci et al. (2008) conducted a review in Foodborne viral infections. Virus extraction and concentration methods was evaluated. Standardized methods for the extraction and concentration of enteric viruses from fresh produce are needed, as current methodologies lack validated consensus protocols.
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