What question did this study set out to answer?

To evaluate matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on linear time-of-flight and magnetic sector instruments for structural characterization of underivatized N-linked glycoprotein oligosaccharides.

December 1, 1994Organic Mass Spectrometry

Examination of complex oligosaccharides by matrix‐assisted laser desorption/ionization mass spectrometry on time‐of‐flight and magnetic sector instruments

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Authors

DHDavid J. HarveyUniversity College Dublin PRPauline M. RuddUniversity College Dublin RBRobert BatemanC-Therm Technologies (Canada)

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Implication

Analytical study demonstrates rapid profiling of underivatized oligosaccharides across diverse glycoproteins, highlighting an efficient alternative to traditional chromatography.

Key Points

To evaluate matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on linear time-of-flight and magnetic sector instruments for structural characterization of underivatized N-linked glycoprotein oligosaccharides.
Analyzed underivatized neutral and sialylated N-linked oligosaccharides from five glycoproteins: ribonuclease B, human IgG, transferrin, bovine fetuin, and chicken ovalbumin.
Tested five distinct chemical matrices, including 2,5-dihydroxybenzoic acid (2,5-DHB), α-cyano-4-hydroxycinnamic acid, and HABA, comparing linear TOF with magnetic sector instrumentation.
Combined MALDI analysis with exoglycosidase enzymatic digests to determine the sequence and branching architecture of human IgG oligosaccharide chains.
2,5-dihydroxybenzoic acid (2,5-DHB) produced the strongest [M + Na]+ signals and the lowest fragmentation for both neutral and acidic sugars after sample recrystallization from ethanol.
The magnetic sector instrument delivered mass resolution up to 3000 FWHM with ~5% relative abundance of informative Y-type glycosidic cleavage fragments, compared to 200–400 resolution on linear TOF.
MALDI-MS completed oligosaccharide mixture analysis within minutes without radiolabelling, significantly outpacing the ~16-hour run times of Bio-Gel P-4 gel filtration chromatography.

Cite This Study

Harvey et al. (1994) studied this question.

synapsesocial.com/papers/6a9014cbc7b7d4543f89d28b https://doi.org/10.1002/oms.1210291208

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Implication

Analytical study demonstrates rapid profiling of underivatized oligosaccharides across diverse glycoproteins, highlighting an efficient alternative to traditional chromatography.

Examination of complex oligosaccharides by matrix‐assisted laser desorption/ionization mass spectrometry on time‐of‐flight and magnetic sector instruments

Discussion

Member takes

Implication

Key Points

Cite This Study

Examination of complex oligosaccharides by matrix‐assisted laser desorption/ionization mass spectrometry on time‐of‐flight and magnetic sector instruments

Discussion

Member takes

Implication

Key Points

Cite This Study

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