What question did this study set out to answer?

To investigate the effects of interleukin-10 on the release of inflammatory mediators by IgE/anti-IgE-activated human cord blood-derived mast cells.

May 1, 2001Clinical & Experimental Allergy

Inhibition of IgE‐induced activation of human mast cells by IL‐10

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Authors

BRBernard RoyerCentre Hospitalier Universitaire de Besançon SVSonia VaradaradjalouCentre National de la Recherche Scientifique PSPhilippe SaasGenVec

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Implication

In vitro study reveals that interleukin-10 inhibits histamine and cytokine release in IgE-activated human mast cells, suggesting therapeutic potential for allergic inflammation.

Key Points

To investigate the effects of interleukin-10 on the release of inflammatory mediators by IgE/anti-IgE-activated human cord blood-derived mast cells.
Differentiated and immunomagnetically purified human cord blood-derived mast cells cultured in the presence of recombinant human stem cell factor and interleukin-6.
Sensitized mast cells with human IgE and challenged them with anti-human IgE with or without varying concentrations of recombinant human interleukin-10.
Quantified levels of histamine, tumour necrosis factor-alpha, interleukin-5, and interleukin-8 in cell supernatants collected at various post-challenge intervals.
Interleukin-10 significantly inhibited the release of tumour necrosis factor-alpha and interleukin-8 from activated mast cells, but exerted no inhibitory effect on interleukin-5 release.
Interleukin-10 suppressed histamine release from activated human mast cells, in direct contrast to earlier observations reported in rodent mast cell models.

Cite This Study

Royer et al. (2001) studied this question.

synapsesocial.com/papers/6a901db1028f4c29a42b163e https://doi.org/10.1046/j.1365-2222.2001.01069.x

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Implication

In vitro study reveals that interleukin-10 inhibits histamine and cytokine release in IgE-activated human mast cells, suggesting therapeutic potential for allergic inflammation.

Inhibition of IgE‐induced activation of human mast cells by IL‐10

Discussion

Member takes

Implication

Key Points

Cite This Study

Inhibition of IgE‐induced activation of human mast cells by IL‐10

Discussion

Member takes

Implication

Key Points

Cite This Study

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