Mechanism of Cycloheximide Inhibition of Protein Synthesis in a Cell-free System Prepared from Rat Liver

Sites of cycloheximideaction on protein synthesis were examined using a cell-free system prepared from rat liver.If all amino acids or aminoacyl transfer RNA were present at the start of incubation, the system appeared to incorporate W-leucine mainly by elongation of peptide chains.Under these conditions, high dose levels of cycloheximide were necessary in order to inhibit incorporation extensively.