September 1, 1999The Journal of General PhysiologyOpen Access

Localization of the K+ Lock-in and the Ba2+ Binding Sites in a Voltage-Gated Calcium-Modulated Channel

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Population

Large conductance Ca2+-activated K+ channel from skeletal muscle incorporated into planar lipid bilayers

Design

Preclinical

Authors

CVCecilia VergaraUniversity of Chile OÁOsvaldo ÁlvarezUniversity of Chile RLRamón LatorreElectrophysiology

Discussion

Member takes

Implication

Findings from this animal model should not yet inform clinical practice; extends structural understanding of the BKCa selectivity filter.

Structured PICO

Population

Large conductance Ca2+-activated K+ (BKCa) channel from skeletal muscle incorporated into planar lipid bilayers

Intervention

Ba2+ as a probe, decreasing external K+ using a K+ chelator ((+)-18-Crown-6-tetracarboxylic acid), and external tetraethylammonium

Outcome

Ba2+ dwell time and channel conductance state

The study identifies that the lock-in site and the Ba2+ sites are the external and internal ion sites of the selectivity filter in BKCa channels, respectively.

Cite This Study

Vergara et al. (1999) studied this question.

synapsesocial.com/papers/6a90221cd27faa463b908fad https://doi.org/10.1085/jgp.114.3.365

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Localization of the K+ Lock-in and the Ba2+ Binding Sites in a Voltage-Gated Calcium-Modulated Channel

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Localization of the K+ Lock-in and the Ba2+ Binding Sites in a Voltage-Gated Calcium-Modulated Channel

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider