Why the study?
Is a two-step real-time PCR assay accurate and reliable for determining capsular serotypes of Haemophilus influenzae compared to conventional PCR and slide agglutination?
Population
131 Haemophilus influenzae clinical isolates from blood, bronchoalveolar lavage fluid, and sputa from…
Comparison
Two-step real-time PCR assay targeting all six… vs Qualitative gel-based PCR assay and slide…
Design
Other
Authors
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Supports real-time PCR as alternative for H. influenzae serotyping; leaves open prospective validation before routine use.
Is a two-step real-time PCR assay accurate and reliable for determining capsular serotypes of Haemophilus influenzae compared to conventional PCR and slide agglutination?
The developed real-time PCR assay is a highly sensitive and specific alternative to conventional PCR and slide agglutination for capsular typing of H. influenzae.
Maaroufi et al. (2007) studied this question.
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