What question did this study set out to answer?

To identify active origins of replication and characterize replication initiation sites in the R plasmid RSF1040 under in vivo conditions.

December 1, 1980Journal of Biological ChemistryOpen Access

Three origins of replication are active in vivo in the R plasmid RSF1040.

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Authors

JCJorge H. CrosaOregon Health & Science University

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Implication

Laboratory analysis reveals three active replication origins in plasmid RSF1040 in vivo, indicating different initiation frequencies compared to in vitro conditions.

Key Points

To identify active origins of replication and characterize replication initiation sites in the R plasmid RSF1040 under in vivo conditions.
Harvested in vivo synthesized replicative intermediate DNA molecules from RSF1040, a deletion derivative of the conjugative plasmid R6K.
Cleaved replicating DNA molecules at a single site using Eco RI restriction endonuclease.
Analyzed the cleaved replicative intermediates using electron microscopy to map replication initiation sites.
Replication initiation occurs at three distinct physical sites designated ori alpha, ori beta, and ori gamma.
The relative activation frequencies among ori alpha, ori beta, and ori gamma in vivo differ distinctly from frequencies previously observed in vitro.

Cite This Study

Jorge H. Crosa (1980) studied this question.

synapsesocial.com/papers/6a9027f3e02ba012352d9f3f https://doi.org/10.1016/s0021-9258(19)70252-x

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Implication

Laboratory analysis reveals three active replication origins in plasmid RSF1040 in vivo, indicating different initiation frequencies compared to in vitro conditions.

Three origins of replication are active in vivo in the R plasmid RSF1040.

Discussion

Member takes

Implication

Key Points

Cite This Study

Three origins of replication are active in vivo in the R plasmid RSF1040.

Discussion

Member takes

Implication

Key Points

Cite This Study

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