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In a previous investigation (Garton, Goodwin & Lijinsky, 1951) it was shown that, using a medium containing 3 % (w/v) glucose, carotene synthesis in Phycomyce8 blakeleeanu8 was independent of the amino-acid used as the nitrogen source with the exception that glycine enhanced carotene produc- tion. Amongst the amino-acids which had no effect was L-valine, from which a hypothetical five-carbon building unit such as P-methylcrotonaldehyde
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Goodwin et al. (1951) studied this question.
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