The properties of a loo-fold purified preparation of rat liver galactose dehydrogenase are presented.Optimum activity occurs at pH 8.3.The enzyme is relatively stable to dialysis, heat treatment, and storage at -30°, but is quite unstable below pH 5. Nicotinamide adenine dinucleotide, but not galactose, protects from heat inactivation.Heat inactivation kinetics suggests that one enzyme is responsible for galactose oxidation.Activity is strongly inhibited by sulfhydryl group inhibitors, C&f, Zn+f, and Fe++, yet sulfhydryl reagents do not enhance activity or protect from inactivation.Activity was not inhibited by cyanide, arsenate, or ethylenediaminetetraacetate.The Km for galactose is 2.6 x 1O-2 M and for NAD is 1.7 X 10d4 M. The V,,, is 0.57 pmole per min per mg of
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Cuatrecasas et al. (1966) studied this question.
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