The oleic acid ester of 4‐methylumbelliferone was found to be a suitable substrate for the fluorimetric assay of the lipase of Geotrichum candidum . This substrate spontaneously formed an emulsion in the assay mixture and had a K m of 10·3 μM. The fluorimetric method was at least a thousand times more sensitive than the conventional potentiometric method. The lower limit of sensitivity of the assay was 1 nmole fatty acid released per min. Optimum conditions for detection of lipase in the culture filtrate of G. candidum were devised. The influence of pH, Ca 2+ and several other factors were investigated.
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DOOIJEWAARD‐KLOOSTERZIEL et al. (1976) studied this question.