Key result
In children developing coeliac disease, the HNRNPL gene was modestly downregulated in peripheral blood B-cells.
Why the study?
In coeliac disease, the functional role of B-cells beyond antibody production has not been analysed extensively.
Cohort
Downregulation of HNRNPL in B-cells may play a role in the development of coeliac disease and susceptibility to enteroviral infections.
Answers are grounded in this paper and cite their sources.
B-cell transcriptomic shifts precede coeliac diagnosis in at-risk children; leaves open HNRNPL's causal role and needs replication before clinical relevance.
Background In coeliac disease (CoD), the role of B-cells has mainly been considered to be production of antibodies. The functional role of B-cells has not been analysed extensively in CoD. Methods We conducted a study to characterize gene expression in B-cells from children developing CoD early in life using samples collected before and at the diagnosis of the disease. Blood samples were collected from children at risk at 12, 18, 24 and 36 months of age. RNA from peripheral blood CD19 + cells was sequenced and differential gene expression was analysed using R package Limma. Findings Overall, we found one gene, HNRNPL , modestly downregulated in all patients (logFC −0·7; q = 0·09), and several others downregulated in those diagnosed with CoD already by the age of 2 years. Interpretation The data highlight the role of B-cells in CoD development. The role of HNRPL in suppressing enteroviral replication suggests that the predisposing factor for both CoD and enteroviral infections is the low level of HNRNPL expression. Funding EU FP7 grant no. 202063, EU Regional Developmental Fund and research grant PRG712, The Academy of Finland Centre of Excellence in Molecular Systems Immunology and Physiology Research (SyMMyS) 2012–2017, grant no. 250114) and, AoF Personalized Medicine Program (grant no. 292482), AoF grants 292335, 294337, 319280, 31444, 319280, 329277, 331790) and grants from the Sigrid Jusélius Foundation (SJF).
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Oras et al. (2023) conducted a cohort in Coeliac disease. Coeliac disease development was evaluated on Differential gene expression in peripheral blood CD19+ B-cells (logFC -0.7, p=q = 0.09). In children developing coeliac disease, the HNRNPL gene was modestly downregulated in peripheral blood B-cells.
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