Key result
A real-time RT-PCR protocol was developed to detect the novel Wuhan betacoronavirus nucleocapsid (N) gene, based on sequence MN908947.
Provides a real-time RT-PCR protocol for detecting the 2019-nCoV nucleocapsid (N) gene.
A real-time RT-PCR to detect the "novel Wuhan" betacoronavirus. Based on sequence MN908947 made available by Professor Yong-Zhen Zhang, Fudan University, Shanghai, China. The target region encodes the nucleocapsid (N). Not tested on wild-type virus (as of 25Jan2020), it is expected to be capable of detecting Wuhan virus, bat-like SARS and SARS virus. Notes: Assay is fully optimised (as of 24Jan2020). A final name for this virus has not been decided (as of 25Jan2020).
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Northill et al. (2020) studied Wuhan coronavirus (2019-nCoV). Real-time RT-PCR N gene assay (Wuhan-N) was evaluated on Detection of the novel Wuhan betacoronavirus nucleocapsid (N) gene. A real-time RT-PCR protocol was developed to detect the novel Wuhan betacoronavirus nucleocapsid (N) gene, based on sequence MN908947.
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