What question did this study set out to answer?

To identify the metabolic pathways and signaling mechanisms governing human eosinophil activation following cytokine stimulation.

December 19, 2019AllergyOpen Access

Interleukin‐5 drives glycolysis and reactive oxygen species‐dependent citric acid cycling by eosinophils

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Authors

NJNicholas JonesSwansea University EVEmma E. VincentUniversity of Bristol LFLindsey C. FelixUniversity of Alberta

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Implication

In vitro study reveals interleukin-5 drives glycolysis and mitochondrial metabolism in human eosinophils, highlighting metabolic plasticity as a potential therapeutic target.

Key Points

To identify the metabolic pathways and signaling mechanisms governing human eosinophil activation following cytokine stimulation.
Isolated peripheral blood eosinophils and treated cells with IL-3, IL-5, or GM-CSF.
Assessed metabolic pathways via extracellular flux analysis, gas chromatography-mass spectrometry with stable isotope tracer analysis, and pharmacological pathway inhibitors.
Human eosinophils primarily rely on glycolysis but also actively recruit mitochondrial metabolism, producing citric acid cycle intermediates from glucose and glutamine following cytokine stimulation.
IL-5-driven metabolic reprogramming operates through the STAT5/PI3K/Akt signaling axis.
Inhibition of NOX-dependent reactive oxygen species increases glucose-derived lactate production, demonstrating functional metabolic plasticity.

Cite This Study

Jones et al. (2019) studied this question.

synapsesocial.com/papers/6a904cc2ccd91641af4dd119 https://doi.org/10.1111/all.14158

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Implication

In vitro study reveals interleukin-5 drives glycolysis and mitochondrial metabolism in human eosinophils, highlighting metabolic plasticity as a potential therapeutic target.

Interleukin‐5 drives glycolysis and reactive oxygen species‐dependent citric acid cycling by eosinophils

Discussion

Member takes

Implication

Key Points

Cite This Study

Interleukin‐5 drives glycolysis and reactive oxygen species‐dependent citric acid cycling by eosinophils

Discussion

Member takes

Implication

Key Points

Cite This Study

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