Summary Pollen grains of taxa representing the four described pollen types of Fagus and Nothofagus were observed with the scanning electron microscope to determine preparation effects. Pollen grains were observed before treatment with KOH, after treatment with KOH, and after acetolysis had been completed. The photomicrographs illustrate that the effect of various preparation techniques differ from species to species. Therefore, it is important in any systematic pollen research to ascertain the amount of change in the material so that the data can be placed in proper perspective for comparative interpretation.
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Hanks et al. (1970) studied this question.