January 1, 1999Thrombosis and Haemostasis

Interspecies Loop Grafting in the Protease Domain of Human Protein C Yielding Enhanced Catalytic and Anticoagulant Activity

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Population

In vitro models of human and bovine activated protein C (APC)

Comparison

Mutagenesis replacing loop 148 in human protein… vs Wild-type human activated protein C and bovine APC

Design

Preclinical

Authors

LSLei ShenYangtze Optical Electronic (China)BVBruno O. VilloutreixInserm BDBjörn DahlbäckMalmö University

Discussion

Member takes

Implication

Supports engineering APC mutants with enhanced activity; leaves open clinical translation from this animal model.

Structured PICO

Population

In vitro models of human and bovine activated protein C (APC)

Intervention

Mutagenesis replacing loop 148 in human protein C with the shorter bovine loop

Comparator

Wild-type human activated protein C (hAPC) and bovine APC

Outcome

Catalytic activity against chromogenic and natural substrates, and anticoagulant activitysurrogate

Replacing loop 148 in human protein C with the shorter bovine loop enhances its catalytic and anticoagulant activity, demonstrating the potential to engineer APC mutants with increased efficacy.

Cite This Study

Shen et al. (1999) studied this question.

synapsesocial.com/papers/6a90648c52891e57888a4aa3 https://doi.org/10.1055/s-0037-1614332

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Interspecies Loop Grafting in the Protease Domain of Human Protein C Yielding Enhanced Catalytic and Anticoagulant Activity

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Interspecies Loop Grafting in the Protease Domain of Human Protein C Yielding Enhanced Catalytic and Anticoagulant Activity

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider