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It has been reported that indiscriminate use of antibacterial compounds in aquaculture has resulted in an increase in populations of antibiotic resistant strains of bacteria. In the present study, Vibrio alginolyticus isolated from infected Penaeus monodon has been found resistant to Furoxone (30 g), Ampicillin (10 g), Cloxacillin (1 g), Bacitracin (10 units), Penicillin (10 units) and Oxytetracycline (30 pg) by antibiotic sensitivity test, suggesting the restricted prophylactic use of antibiotics in shrimp aquaculture industry. As an approach towards differentiation and identification of bacterial strain, the plasmid characterization of V. alginolyticus by restriction enzyme analysis was carried out which revealed the presence of 6.3 kb and 0.8 kb DNA fragments by Hind lil and a 7. 9 kb DNA fragment by EcoRI enzymes. The cell envelope characterization of V. alginolyticus by SOS-PAGE showed the presence of 12 protein bands in the range of 29 to 112.20 kD. The preliminary information on the cell envelope characterization may help in studies relating towards development of immunostimulant against vibriosis.
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Shekhar et al. (2023) studied this question.
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