Caco-2 cells are more effective than RD and L20B cells for the isolation of enteroviruses from environmental sewage samples, especially when using trypsin treatment.
Caco-2 cells may improve environmental enterovirus surveillance; leaves open validation for clinical diagnostics or broader programs.
The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in the inoculation process.
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Wieczorek et al. (2013) studied this question.
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