Why the study?
Does an integrated cell culture-reverse transcriptase PCR approach reduce the time to detect infectious enteroviruses in environmental samples compared to cell culture alone?
Does an integrated cell culture-reverse transcriptase PCR approach reduce the time to detect infectious enteroviruses in environmental samples compared to cell culture alone?
An integrated cell culture-PCR procedure enables rapid detection of infectious enteroviruses in environmental samples within 24 hours, overcoming limitations of direct RT-PCR.
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May accelerate enterovirus detection in environmental samples; leaves open field validation before routine public-health adoption.
Rapid detection of infectious enteroviruses in environmental samples was made possible by utilizing an integrated cell culture-reverse transcriptase PCR approach. By this method, the presence of infectious enterovirus was confirmed within 24 h, compared with > or = 3 days by cell culture alone. The combined methodology eliminated typical problems normally associated with direct reverse transcriptase PCR by increasing the equivalent volume of environmental sample examined and reducing the effects of inhibitory compounds.
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Reynolds et al. (1996) studied this question.
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