Why the study?
Does nested PCR combined with RFLP improve the detection and rapid differentiation of human enteroviruses compared to routine virus isolation and neutralization?
Population
32 different serotypes of prototypic strains of human enterovirus, and uncultured specimens from patients…
Comparison
Nested PCR with specific primers derived from… vs Routine method of enterovirus diagnosis based on…
Design
Preclinical
Authors
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Should not yet change clinical testing; hypothesis-generating for rapid enterovirus detection in humans.
Does nested PCR combined with RFLP improve the detection and rapid differentiation of human enteroviruses compared to routine virus isolation and neutralization?
Nested PCR combined with RFLP provides a highly sensitive and rapid method for detecting and differentiating human enteroviruses directly from clinical specimens.
M.-M. Kuan (1997) studied this question.
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