Key result
Real-time nested PCR detects enterovirus in ~86% of suspected aseptic meningitis cases, outperforming viral culture.
Why the study?
Conventional methods for laboratory diagnosis of enterovirus infections have limitations in sensitivity and speed, motivating evaluation of a new rapid detection approach.
Does real-time nested PCR improve the detection rate of enterovirus in CSF compared to conventional PCR and culture in patients suspected of aseptic meningitis?
Population
50 CSF specimens from patients suspected of aseptic meningitis
Comparison
Real-time nested PCR vs PCRs for 3 targets and enterovirus culture
Design
Cross-sectional study
Authors
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Real-time nested PCR may speed enterovirus detection in CSF; leaves open routine adoption pending larger validation.
Cross-Sectional (n=50)
Does real-time nested PCR improve the detection rate of enterovirus in CSF compared to conventional PCR and culture in patients suspected of aseptic meningitis?
Absolute Event Rate: 86% vs 6.8%
Real-time nested PCR provides a highly sensitive and rapid method for diagnosing enterovirus infections in cerebrospinal fluid.
Heo et al. (2006) conducted a cross-sectional in Suspected aseptic meningitis (n=50). Real-time nested PCR vs. Virus culture and standard PCR was evaluated on Positive detection rate of enterovirus. Real-time nested PCR detected enterovirus in 86.0% of CSF specimens from patients with suspected aseptic meningitis, compared to 6.8% by viral culture.
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