In situ hybridization using a biotinylated cDNA probe can successfully detect Coxsackie virus B4 RNA in murine tissues, demonstrating potential utility for analyzing human biopsy samples.
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Validates in situ hybridization for pancreatic viral RNA detection in mice; leaves open utility for human biopsy analysis.
The pathology of Coxsackie virus B4 (CVB4) infection in a murine model was investigated by in situ hybridisation using a biotinylated cDNA probe derived from CVB4. During the acute phase of infection virus RNA sequences were detected in the exocrine pancreas of 60% of mice infected with a pancreotropic variant of CVB4. A positive hybridisation signal was observed in other organs in some animals including the heart and liver of 1 mouse 28 days after infection. The cellular distribution of virus RNA sequences corresponded well with the histological findings in most tissues. Possible causes for failure of hybridisation in some infected pancreases are discussed in conjunction with potential application of the technique in human pancreas biopsy samples.
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Vella et al. (1991) studied this question.
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