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Data are presented to compare the size separation capabilities of several porous packing systems for latex particle chromatography. Material balance problems as well as nonequilibrium behavior encountered with small‐pore systems (pores less than 1 μm) lead to the conclusion that larger pore systems (diameter 2 μm or greater) are superior from an operational viewpoint. The separation mechanism for a large‐pore Fractosil system is shown to be predominantly hydrodynamic in nature with flow separation occurring in both the pores and packing interstices. The name Hydrodynamic Permeation Chromatography, or HDPC, is used to describe the process. The effects of calibration slope and band broadening on peak resolution are compared for an HDC system and the Fractosil system indicating the superior resolving power of the HDC system. Qualitative and semiquantitative considerations are made with regard to improved porous packing characteristics for an ideal HDPC system which would improve separation efficiency and resolution.
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Nagy et al. (1981) studied this question.
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