Association of AGTR1 (A1166C) and ACE (I/D) Polymorphisms with Breast Cancer Risk in North Indian Population

Figures

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  Figure 1. Determination of AGTR1 (A1166C) and ACE (I/D) polymorphism by PCR-RFLP. a The agarose gel picture represents the DNA fragment bands obtained after DdeI restriction enzyme digestion for control population. The DNA ladder marker is represented by M1. Lanes C1 and C2 represent the presence of A1166C SNP; both the lanes have heterozygous AC with 530 bp, 412 bp and 118 bp bands. Lanes C3, C4, C5, C6 have homozygous AA with 530 bp band. The bar graph represents percentage distribution of the AA, AC and CC genotypes in control population. b The agarose gel picture represents the data for BCa patient population. Lanes B3 and B4 represent the presence of A1166C SNP; both the lanes have heterozygous AC with 250 bp, 129 bp and 121 bp bands. Lanes B1, B5, B6 have homozygous AA with 250 bp band. The bar graph represents percentage distribution of the AA, AC and CC genotypes in BCa patient population. c The representative agarose gel picture depicts the ACE (I/D) polymorphism for control population. Lanes A1, A2, A5, A6 depict the DD genotype as indicated by the 190 bp DNA fragment band. Lanes A3 and A4 depict the II genotype as shown by the presence of 490 bp DNA band. The bar graph…

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  Figure 2. Epistatic interaction between AGTR1 (A1166C) and ACE (I/D) polymorphisms using MDR. The black bar in each cell represents frequency of BCa patients' cases and white bar represents frequency of healthy individual controls. High risk genotype combinations are represented by dark gray shade cells, while light gray shade cells represent low risk genotype combinations. Cells with no shading or white cells represent genotype combination for which no data is observed.

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  Figure 3. Illustration showing genotypic combinations of AGTR1 (A1166C) and ACE (I/D) polymorphisms and their respective odds ratio for breast cancer risk.

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  Supplementary Figure S1. Representation of AGTR1(A1166C) SNP by Sanger sequencing and SNP analysis data. a Chromatogram image of DNA sequencing data for control population. The image represents A to C polymorphism in control population. b Representative image of SNP analysis in control population for AGTR1(A1166C) SNP. c Same as a, except for BCa patient population. d Same as b, except AGTR1(A1166C) SNP for BCa patient.