What question did this study set out to answer?

To determine whether Arabidopsis thaliana At-NHX1 and yeast NHX1 encode functional vacuolar Na+/H+ exchangers and to examine their structural and pharmacological features.

September 26, 2000Biochemical Journal

Arabidopsis thaliana and Saccharomyces cerevisiae NHX1 genes encode amiloride sensitive electroneutral Na+/H+ exchangers

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To determine whether Arabidopsis thaliana At-NHX1 and yeast NHX1 encode functional vacuolar Na+/H+ exchangers and to examine their structural and pharmacological features.
Heterologously expressed Arabidopsis thaliana At-NHX1 to functionally complement a Saccharomyces cerevisiae nhx1-null mutant.
Isolated vacuolar vesicles from transformed yeast to measure pH-gradient-driven Na+ accumulation and evaluated the functional impact of adding an N-terminal signal peptide.
Tested transport sensitivity to the diuretic compound amiloride across yeast and plant antiporters.
Both At-NHX1 and NHX1 functioned as low-affinity electroneutral Na+/H+ exchangers facilitating pH-gradient-energized vacuolar sodium sequestration.
Fusion of the yeast N-terminal signal peptide to At-NHX1 increased the overall magnitude of Na+/H+ exchange activity.
At-NHX1 activity was completely inhibited by amiloride, whereas yeast NHX1 activity was inhibited by only 20% to 40%.

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Implication

Experimental study demonstrates vacuolar sodium-proton exchange by plant and yeast NHX1 proteins in yeast models, indicating distinct structural regulation and amiloride sensitivity.

Cite This Study

DARLEY et al. (2000) studied this question.

synapsesocial.com/papers/6a909378b71d06dae589f12f https://doi.org/10.1042/bj3510241

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Implication

Experimental study demonstrates vacuolar sodium-proton exchange by plant and yeast NHX1 proteins in yeast models, indicating distinct structural regulation and amiloride sensitivity.

Arabidopsis thaliana and Saccharomyces cerevisiae NHX1 genes encode amiloride sensitive electroneutral Na+/H+ exchangers

Key Points

Discussion

Implication

Cite This Study

Arabidopsis thaliana and Saccharomyces cerevisiae NHX1 genes encode amiloride sensitive electroneutral Na+/H+ exchangers

Key Points

Discussion

Implication

Cite This Study

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