Acid ensilage treatment preserves crawfish heat‐processed waste under ambient temperature conditions with stabilization of the astaxanthin pigment present. Implementation of acid ensiling prior to pigment extraction increased concentration of the astaxanthin oil extract by 40–50%, and oil recovery by 10%. A twofold increase in free amino nitrogen, and a 70% reduction in exoskeleton calcium carbonate, were observed in crawfish silage (pH 4.2) from acid/enzymatic hydrolysis, compared with controls. A correlation was seen between solubilization of calcium carbonate and pigment release in relation to silage pH. The role of acid‐resistant proteolytic microorganisms in breakdown of carotenoprotein complex is discussed. Application of ensilage process for ultimate commercial production of astaxanthin‐enriched vegetable or fish oils from pigment rich crustacean wastes is postulated.
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CHEN et al. (1983) studied this question.
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