Micro high performance liquid chromatography of aliphatic amines by means of resonance raman detection

A micro high performance liquid chromatography coupled with a resonance Raman detection system is described. For highly sensitive Raman detection, aliphatic amines were derivatized with dabsyl chloride (4‐dimethylaminoazobenzene‐4′‐sulfonyl chloride). The derivatives were separated on an ODS micro column (0.5 mm i.d. × 145 mm PTFE tube). Chromatograms were obtained by measuring the intensity of Raman scattering at 1136 cm−1 with the 488.0 nm line of an Arion laser. The lower detection limit was 1.5 ng and the RSD of relative peak height (n = 9) was 5.9% at 11 ng of methylamine derivative. Moreover, by stopping the flow of the micro HPLC system at the retention time of the individual derivatives, it was possible to measure their resonance Raman spectra.