The isolation and characterization of a cupro- protein, erythrocuprein, from normal human erythrocytes have been described recently (1-3). Preliminary studies indicated that normal human erythrocytes contain about 30 to 36 mg of erythro- cuprein per 100 ml of packed cells (1). Since erythrocytes are known to hold about 100 ,ug of copper per 100 ml of packed cells (4), and since erythrocuprein carries 3.4 /Ag of copper per mg of protein, we suggested that erythrocuprein ac- counts for most of the copper in erythrocytes.
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Shields et al. (1961) studied this question.