A semisynthetic vesicular membrane was constructed by immobilization of a synthetic polyelectrolyte [poly(2-ethylacrylic )] on the surface of a phosphatidylcholine bilayer. Immobilization was accomplished via Michael addition of polymer-bound functions to a reactive maleimide handle incorporated into the lipid membrane in the form of dimyristoyl-N-[ [4-(maleimidomethyl)cyclohexyl]carbonyl]phosphatidylethanolamine. membranes prepared in this way are sensitive hydrogen ion concentration and are subject to large variations in permeability with small changes in pH. Rapid and quantitative of vesicle contents can be achieved by mild acidification within the physiological pH range.
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Maeda et al. (1988) studied this question.