Protein Quality Control Mechanisms and Protein Storage in the Endoplasmic Reticulum. A Conflict of Interests?

More than 30 years ago it was observed that the storage proteins of maize (Zea mays) seeds accumulate as “bulges or localized dilatations along the endoplasmic reticulum cisternae” of developing endosperm cells (Khoo and Wolf, 1970). It later became evident that, whereas the widespread seed storage proteins of the 7S and 11S classes travel from the endoplasmic reticulum (ER) to the Golgi complex and are then deposited in vacuoles, a number of cereal storage proteins instead form electron-dense, round-shaped structures with diameters of 0.5 to 2.0 μm, termed protein bodies, within the ER lumen (Herman and Larkins, 1999). These large aggregates are then either permanently stored in the ER or delivered to storage vacuoles by unconventional protein traffic pathways. Today, the mechanisms by which some of the most important proteins for human nutrition form protein bodies within the ER remain a fascinating but still puzzling issue in cell biology. This developmentally programmed use of the ER to store vast amounts of specific proteins in highly condensed forms has been found only in plants. On the other hand, aggregation of newly synthesized proteins in the ER, due to stress or genetic defects, is usually treated by the cell as a pathology that must be avoided by disposing of the misfolded proteins (Sitia and Braakman, 2003). Many ER resident proteins indeed have the role of preventing protein aggregation, maintaining nascent and newly synthesized polypeptides in a state that is compatible with further structural maturation or, for defective proteins, with degradation. Thus, proteins destined for storage in the ER must have evolved to condense in a controlled fashion and thus to avoid both export from the ER and degradation. How this might be achieved is the topic of this update. The ER is part of the endomembrane system, which also includes the Golgi complex, vacuoles, and the plasma membrane as major components. The system hosts the secretory pathway, which synthesizes and delivers to the correct location most of the proteins of the above-mentioned compartments and of the cell wall. These proteins are collectively termed secretory proteins. The ER plays the role of a protein nursery, assisting in the folding and assembly of newly synthesized secretory proteins before they traffic to the Golgi complex and then the vacuoles or the cell surface (Vitale and Denecke, 1999). Many residents of the ER have signals that promote their localization in this compartment (Vitale and Denecke, 1999), but storage proteins that accumulate within the ER do not carry any of these known signals. How do they form stable structures in the ER? To try answering, we need to take into consideration many aspects of ER functions and their regulation. Sequencing of plant genomes shows that thousands of genes encode proteins with signals for insertion into the secretory pathway; consistently, the ER is very well equipped to assist in the folding and assembly of newly synthesized secretory polypeptides (Vitale and Denecke, 1999). Indeed, it seems that, directly or indirectly, most ER residents are helpers of protein folding. Proteins that fail to undergo correct conformational maturation are dislocated from the ER into the cytosol or delivered to vacuoles and then degraded. The promotion of protein folding and the disposal of defective proteins are strictly related and are collectively termed quality control (Vitale and Denecke, 1999; Sitia and Braakman, 2003). A schematic view of quality control. Secretory proteins emerge cotranslationally into the ER lumen. Nascent chains undergo intermediate steps of structural maturation. During this process they are retained in the ER. Once a protein has folded and, in the case of multimeric proteins, assembled, it is available for traffic to its final destination. Proteins with permanent defects are retained in the ER for prolonged periods of time (or rapidly retrieved from the Golgi complex) and often eventually dislocated into the cytosol for degradation; alternatively, they accumulate as aggregates in the ER. In plant cells, a number of proteins are naturally retained and stored permanently or transiently in the ER as large assembled/aggregated structures. However, some native or artificial storage proteins accumulated as protein bodies in the ER also associate for a long time after synthesis with the plant homologue of the immunoglobulin heavy chain binding protein (BiP), which is the ER member of the heat shock 70 chaperone family (Li et al., 1993; and in this issue Mainieri et al., 2004). Persistent interactions with the ER helpers are therefore not sufficient per se for degradation, and this seems to constitute a second paradox, at least in the cells that accumulate storage proteins in the ER. Storage proteins accumulating in the ER must therefore be able to take advantage of the folding machinery of this compartment to reach a conformational state that is not compatible with export and that, at the same time, does not direct the protein to the degradative phase of quality control. To understand how this can be achieved we first need to examine the mechanisms of protein export from the ER. Export from the ER requires the recruitment of a specific coat protein complex, termed COPII, on the cytosolic face of the ER membrane, and it is thought to occur at the level of a subcompartment (transitional ER) defined by the presence of COPII components (Barlowe, 2003). This forward movement is balanced by backwards recycling traffic that retrieves membrane lipids and specific proteins and uses a different coat, termed COPI. Two nonexclusive models have been proposed for the export of soluble proteins (Vitale and Denecke, 1999; Barlowe, 2003). According to the first, the bulk-flow model, proteins are not concentrated at ER exit sites and diffuse in a passive fashion into COPII vesicles. Concentration of secretory cargo would not occur at the exit of the ER, but rather by exclusion from the COPI vesicles originating from the Golgi complex that recycle ER proteins. According to the second model, receptor-mediated mechanisms allow cargo concentration into COPII vesicles, and evidence has been collected indicating that membrane proteins ERGIC53, Emp24p, and Erv29p are implicated in the recognition of certain soluble proteins and in their export from the ER (Barlowe, 2003). These receptors must be able to bind sorting signals present in soluble secretory proteins and to actively recruit COPII. These signals may be concealed in misfolded proteins, and this could contribute to their retention in the ER. Retention of misfolded proteins may also be due to their interaction with ER folding factors. ER chaperones and folding helpers often contain signals (such as the KDEL/HDEL motif in soluble ER residents and the dilysine/diarginine motif in membrane residents; Vitale and Denecke, 1999) that are recognized in the Golgi complex and promote retrieval into the ER by COPI vesicles. Certain misfolded proteins can thus be retrieved from the Golgi complex by virtue of their association with a recycling chaperone. Chaperones may also be retained because they form an insoluble protein matrix that effectively reduces their inclusion in budding COPII vesicles. It would seem reasonable to assume that binding to such a resident protein matrix could effectively hamper the export of unfolded proteins from the ER (Sitia and Braakman, 2003). In conclusion, depending on the protein and on the mechanism of export, the lack of (exposed) export signals in misfolded proteins, together with the binding to molecular chaperones in the ER and to retrieval systems, can contribute to quality control. One additional mechanism of protein retention in the ER is mediated by the formation of protein aggregates. In this case, the intrinsic biophysical properties of the protein lead to the formation of complexes that are unable to be inserted in the lumen of COPII vesicles. The accumulation of protein aggregates in the ER is normally induced by stress conditions or by the expression of defective proteins. In these situations, the degradative capacity of the ER may be insufficient to take care of the bulk of misfolded proteins, which may then be segregated into subregions of the ER (Sitia and Braakman, 2003). Artificial overaccumulation of protein in the ER does not always result in the same alteration of ER morphology. When developing lima bean cotyledons are treated with tunicamycin (A), the storage proteins destined for storage vacuoles aggregate and the ER cisternae (arrows) enlarge, maintaining an electron-transparent aspect. When zeolin, a chimeric protein composed of phaseolin and γ-zein domains, is expressed in tobacco leaves (B), it forms round-shaped, electron-dense protein bodies within the ER. Immuno-gold labeling in A, performed with anti-7S storage protein antiserum, decorates the storage protein already accumulated in storage vacuoles before the tunicamycin treatment, as well as some aggregates within the ER. Immuno-gold labeling in B, performed with antiphaseolin antiserum, decorates the protein body. PB, Protein body; PSV, protein storage vacuole; V, vegetative vacuole. Bars = 1 μm. For more details on these experiments see Sparvoli et al. (2000) and Mainieri et al. (2004). Depending on the protein, individual chaperones can be included or excluded from the aggregates, but sequestration of excessive amounts of folding helpers in these aggregates is likely to be detrimental to the cell. Consistent with this view, it has been proposed that BiP sequestration into aggregates can lead to a reduction of free BiP to a level that cannot be tolerated by a yeast (Saccharomyces cerevisiae) strain unable to respond to ER stress (Umebayashi et al., 1999). Storage proteins accumulating in the ER may take advantage of any of the above-mentioned retention or retrieval mechanisms and use controlled aggregation as a way to escape the final degradative phase of quality control and accumulate in the ER lumen without exposing this compartment to an excessive level of stress. Protein body formation has been extensively studied in maize. The protein bodies that form in the ER of maize endosperm cells contain a set of polypeptides that are classified as α-, β-, γ-, and δ-zeins. Individual β- or γ- zeins can form stable protein body-like accretions in the ER of transgenic plants in the absence of other subunits (for review, see Herman and Larkins, 1999), but it is clear that formation of protein bodies in maize normally involves both homotypic and heterotypic interactions (Kim et al., 2002). The latter have been shown to be important for the stability of α- and δ-zeins in transgenic tobacco (Nicotiana tabacum) plants. The accumulation of these two proteins is highly increased when they are coexpressed with γ-zein or β-zein, respectively (Herman and Larkins, 1999). Interestingly, γ- and β-zeins are synthesized before the α- and δ- polypeptides during maize seed development, supporting the hypothesis of their fundamental role in the formation of a stable protein body. Possible mechanisms of protein body biogenesis. Newly synthesized polypeptides destined for storage in the ER interact with chaperones and folding helpers, similarly to the other secretory proteins. These interactions are, however, persistent. Specific domains may also interact directly with the lipids of the ER membrane. The possible relationships between the two events are unknown, and both may contribute to retention in the ER until protein bodies are formed. The involvement of each of these processes is likely to vary between different protein body proteins. The mature protein bodies are too large to enter COPII vesicles and are permanently retained in the ER, or transported to the vacuole by alternative pathways. Chaperones like BiP may remain present on the surface of the protein body, to assist the late steps of assembly and avoid uncontrolled exposure of hydrophobic domains that could negatively affect ER functions. A region necessary for retention within the ER has been identified in a γ-zein polypeptide by mutagenesis and expression in transgenic Arabidopsis (Arabidopsis thaliana) plants. This polypeptide consists of a Pro-rich tandem repeat domain, followed by a linker region and by a C-terminal Cys-rich domain. While the wild-type protein is retained by Arabidopsis leaf protoplasts and localizes to protein bodies from which BiP is largely excluded, a deletion mutant lacking the repeat and linker domains is secreted (Geli et al., 1994). These results strongly suggest that retention in the ER is not simply due to the absence of an active export signal and implicate the repeat domain in retention. Interestingly, a truncated version of the protein consisting of the repeat and linker domains accumulates in enlarged regions of the ER of Arabidopsis cells that can also be stained with an anti-BiP antiserum (Geli et al., 1994). Whether these sequences contain a BiP-binding site remains to be determined, but these data would be compatible with the view that the N-terminal region of the γ-zein protein may mediate retention within the ER by first binding to BiP and then driving protein body formation. Consistently, when the repeat and linker regions of γ-zein are appended to the trimeric vacuolar protein phaseolin, the fusion protein, termed zeolin, is retained in the ER and stably deposited in protein body-like structures (Fig. 2B; see Mainieri et al., 2004). These protein bodies sequester a proportion of BiP, which can be released by ATP in vitro treatment, indicating that BiP is not trapped but is acting as a chaperone (heat shock-70 chaperones are ATPases and use ATP hydrolysis to perform their function). Zeolin protein bodies are insoluble and can be solubilized and disassembled by reducing agents (Mainieri et al., 2004). These results indicate that disulfide bonds are a key factor in zeolin protein body assembly and that the process is extensively assisted by BiP. Further work directly addressing the question whether BiP or other chaperones bind the N-terminal domain of γ-zeins within the context of the native protein will be necessary to clarify the mechanism by which the repetitive domain drives retention in the ER. As mentioned above, a role can also be played by direct interactions with lipids because a synthetic version of the repetitive domain of γ-zein strongly interacts with liposomes in vitro (Kogan et al., 2004). γ-Zeins belong to the prolamin superfamily of storage proteins, which has representatives in a range of cereals such as wheat (Triticum aestivum), oats (Avena sativa), and rice (Oryza sativa). The rice prolamins do not contain a repeated sequence, but their deposition has been shown to be assisted by the chaperone BiP (Li et al., 1993). BiP is associated to the surface of rice prolamin protein bodies (Muench et al., 1997) and has been proposed to be necessary to maintain the prolamins in a competent state for subsequent assembly in the ER. In wheat endosperm, BiP is found within the prolamine protein bodies rather than bound to their surface, possibly suggesting that this chaperone plays different functions in the biogenesis of protein bodies in different species (Levanony et al., 1992). It is also worth noting that proteins with similar primary structure may be differently recognized by the ER quality control system and that the level of export competency of a given protein may be dependent on the environment encountered within the ER lumen. In wheat, the sulfur-rich prolamins include both low Mr (LMW) glutenin subunits and γ-gliadins. The two classes of proteins share the same domain architecture, with an N-terminal repetitive domain rich in Gln and Pro, and a C-terminal domain stabilized by a set of conserved disulfide bonds. However, while gliadins are mainly monomeric, LMW glutenin subunits assemble into disulfide-linked polymers. Individual members of these two classes of proteins have different intracellular fates when expressed in Xenopus oocytes. While a γ-gliadin polypeptide is in large part export competent, an LMW glutenin subunit is instead fully retained, possibly at the level of the ER (Altschuler and Galili, 1994). The mechanism(s) that mediate the complete retention of the LMW glutenin subunit remain unclear, but structural features within the C-terminal nonrepetitive domain may be involved (Altschuler and Galili, 1994). It is interesting to note that the γ-gliadin polypeptide was found to be transport competent also when expressed in transgenic tobacco plants (Napier et al., 1997), but it was found to form protein body-like structures in the yeast ER (Rosenberg et al., 1993). The cell type-specific destiny of a γ-gliadin when expressed in heterologous systems may reflect differences in the ER machinery or in the concentration reached by the newly synthesized protein in the ER lumen. Indeed, protein assembly is a concentration-dependent process and can be dramatically affected by changes in the rate of protein synthesis (Ceriotti et al., In the native interactions with other or other might the of to the ER, as by the of Golgi complex in wheat endosperm cells during the of storage protein synthesis and deposition (Levanony et al., 1992). The role of or interactions with prolamins in the final destiny of a polypeptide after into the ER is also by the that a γ-zein is into native protein bodies when expressed in maize endosperm its wild-type in part secreted in transgenic Arabidopsis plants et al., The and of the ER are not in different as for by the ER during seed development, and are also by stress or proteins are secretory interesting issue of cell is how the ER its functions to increased of secretory proteins or to the stress by of protein folding. This process has been termed unfolded protein and has been more studied in yeast and in cells and 2004). has relationships with quality control (Sitia and Braakman, and 2004). The major seems to be by BiP. its interactions with newly synthesized secretory proteins, in cells BiP also more permanently proteins that in the and and 2004). for any the of BiP in the ER the chaperone is from these proteins to involved as a folding As a and and is transported to the Golgi These events a of in the specific of genes that encode folding helpers of the ER, to a of protein these do not in the can eventually be the cell the of BiP that is not as a chaperone. defective proteins into the ER undergo interactions with BiP before for degradation, and therefore they a proportion of BiP from the and 2004). A of of the ER, as a result of programmed or in the expression of secretory protein can also have the same many secretory proteins are and because is often important for protein folding and tunicamycin is a and of tunicamycin is not a of during the of a It is therefore interesting to other in which increased of ER folding helpers have been BiP and protein disulfide are in storage cells of developing to vegetative et al., et al., et al., In developing could be due to of BiP from the ER because of its as a chaperone on the newly synthesized storage proteins. However, in wheat endosperm the of protein disulfide are already before storage protein accumulation can be by protein et al., This that, at least is of the known during of the of BiP before that of proteins et al., 1999). A on the changes when cells into cells the plant of the ER machinery immunoglobulin et al., 2003). It is reasonable to that the synthesis of storage or proteins has ER further but the first of ER must be by than the of these proteins into the ER. In this the that is induced by a number of endosperm maize is important (Herman and Larkins, 1999; et al., 2002). The in directly affect structural The and have been extensively a of the signal in the two affected proteins (Herman and Larkins, 1999; et al., 2004). in of the level of wild-type maize still has of indicating that protein body formation in not an aggregation process and that storage proteins are not treated as defective proteins. This the observed in cells proteins to form aggregates in the ER et al., protein body formation is observed in the and maize and 1992). the of protein bodies could be directly to the presence of the an alternative and is that chaperone due to with the process of protein body formation. The of chaperone in a protein assembly process is by the that the artificial of BiP transiently protein assembly in the plant ER et al., 2003). The synthesis of large amounts of secretory proteins requires at the level and a between pathways. performed during the has on the way these processes are and the mechanisms that allow the cell to with the heavy encountered during the of storage protein In some plant cells have the of the ER to directly accumulate the synthesized polypeptides without the need for further transport along the secretory this process could be as the aggregation of insoluble it is clear that specific of the accumulated proteins and specific interactions with must be important to allow the deposition of the storage proteins without the ER to an level of stress. The details of these processes have in some but many still remain The role of the in the process to ER during seed to be in more The of of storage protein synthesis for the formation of protein bodies is an issue that be The widespread of disulfide bonds in cereal storage proteins for a more of their role in the The for more of protein body formation can also lead to the of protein maturation The question whether are regions of the ER protein body formation can remains these understand not only the mechanism of storage protein deposition but also how cells in to with proteins into the ER. and for the in A and B, the many with we have during the years of the in this and we to work could not be due to