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Although both NEM and IA completely inhibited G3PD activity and decreased RBC ATP levels 40%, IA produced little effect on RBC filterability whereas NEM greatly reduced the filtration rate. IA caused only an isolated increase of band 6 (G3PD) and no change in membrane protein content. However, the decreased filter-ability of NEM-treated RBC was associated with significantly increased membrane-associated nonhemoglobin protein which appeared as a complex array of polypeptide bands on PAGE SDS.
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Allen et al. (1976) studied this question.
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