Recent emergence of a novel porcine pestivirus: interference with classical swine fever diagnosis?

Figure 1. Immunostaining of APPV- and CSFV-infected porcine kidney cells. APPV genome-positive cells (11th passage of SPEV cells) were positively stained with a porcine field serum (diluted 1:1000; goat α-swine IgG, Cy3-labeled, Dianova, #114-165-003, 1:250) that was also reactive against the recombinantly expressed APPV Erns. The same passage of APPV-positive cells was not detected by the broadly reactive pestivirus NS3-specific mAb 8.12.7 (diluted 1:20; goat α-mouse IgG, Cy3-labeled, Dianova, #115-165-003, 1:800) or mAb C16 (diluted 1:25; goat α-mouse IgG, Cy3-labeled, Dianova, #115-165-003, 1:800). The APPV-specific field serum did not stain the CSFV-infected SK6 cells.