Induction of Isoantibodies to Human Leukocytes

As an exploratory first step toward the production of leukocyte antisera of circumscribed and predictable specificity, 10 volunteers were immunized, each to the leukocytes of a single donor. Washed leukocyte suspensions were injected i.d. and i.m. and in four instances, transfusions were given. All the volunteers developed leukoagglutinins and complement-fixing antibodies to leukocytes in 40–293 days. These antibodies persisted for 72–223 days after the final injection. Seven of the 10 developed complement-fixing antibodies to the donors' platelets. The induced antisera have been used for leukocyte grouping of other normal subjects.