January 1, 1985Cold Spring Harbor Symposia on Quantitative Biology
Elastase I Promoter Directs Expression of Human Growth Hormone and SV40 T Antigen Genes to Pancreatic Acinar Cells in Transgenic Mice
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Discussion
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Implication
Experimental study demonstrates targeted gene expression in pancreatic acinar cells using an elastase I promoter in transgenic mice, highlighting a mechanism for cell-specific pancreatic targeting.
Key Points
- To determine whether the elastase I promoter can selectively direct foreign gene expression specifically to pancreatic acinar cells in transgenic mice.
- Engineered transgenic mice harboring fusion gene constructs linking the elastase I promoter to human growth hormone and SV40 T antigen genes.
- Evaluated tissue specificity and developmental timing of transgene expression across pancreatic cell lineages and other organ systems.
- The elastase I promoter directed targeted transgene expression exclusively to pancreatic acinar cells, sparing endocrine cells and non-pancreatic tissues.
- Targeted expression followed the endogenous developmental timeline of digestive serine proteases, which rise sharply prior to birth and achieve adult levels postnatally.
Cite This Study
Ornitz et al. (1985) studied this question.