Further Studies on the Rna Functional in Auxin Action

Avena coleoptile and a respiration-deficient mutant of yeast whose cells are elongated by the action of auxin in the absence of GA added were subjected to the phenol method of RNA fractionation. “RNA” from the phenol layer promoted the auxin-induced expansion of Jerusalem artichoke tuber slices just as GA did, whereas “RNA” from the water layer was ineffective. The GA-like activity of the former “RNA” was reduced by treatment with RNase or alkali. No active “RNA” was extractable from a respiration-sufficient yeast strain which does not respond to auxin without GA supplemented.