A previously described optical technique for enhancing interference colour contrast in polarized light microscopy is adapted for quantitative image analysis. This is based on a linear relationship for colour intensity vs. molecular birefringence and degree of phase alignment, derived using nematic liquid crystal approximations, and verified experimentally. The image analysis is described and illustrated on a section of the marginal rib of the dogfish egg case.
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Ross et al. (1997) studied this question.