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May 1, 1979Journal of Biological ChemistryOpen Access

Identification of in vitro synthesized pituitary glycoprotein alpha subunit. Translation of a possible precursor.

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Population

Bovine pituitary RNA translated in heterologous cell-free systems derived from wheat germ and reticulocyte…

Design

Preclinical

Authors

TLThomas D. LandefeldUniversity of Michigan

Discussion

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Implication

Hypothesis-generating for LH alpha-subunit biosynthesis; leaves open translation to human glycoprotein hormone regulation.

Structured PICO

P
Population
Bovine pituitary RNA translated in heterologous cell-free systems derived from wheat germ and reticulocyte lysate
I
Intervention
In vitro translation and immunoprecipitation with specific antisera (bovine lutropin alpha antisera, bTSH beta, bLH beta)
O
Outcome
Identification and molecular weight characterization of synthesized pituitary glycoprotein subunits

The study demonstrates that the pituitary glycoprotein alpha subunit is synthesized as a larger precursor molecule separately from the beta subunit.

Cite This Study

Thomas D. Landefeld (1979) studied this question.

synapsesocial.com/papers/6a910507f61bbdbd5ac1b144https://doi.org/10.1016/s0021-9258(18)50636-0
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Nascent prehormones are intermediates in the biosynthesis of authentic bovine pituitary growth hormone and prolactin.1977 · 201 citations
  2. 2A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels1974 · 8,651 citations
  3. 3The amino acid sequence of human chorionic gonadotropin. The alpha subunit and beta subunit.1975 · 294 citations
  4. 4Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes.1978 · 215 citations
  5. 5Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat Germ1973 · 1,210 citations