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The Fisher's dispersion analysis test was employed to establish the accuracy limits of spermatozoa concentrations for the evaluation of ATP content of spermatozoa. It was established that the Bücher's method working between 4 times 108 and 1 times 108 spermatozoa, showed no significant variation of the results. With less than 1 times 108 spermatozoa results are significantly different and therefore they are not reliable.
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Calamera et al. (1979) studied this question.
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