The association on SDS-polyacrylamide gels of lipopolysaccharide and outer membrane proteins ofPseudomonas aeruginosaas revealed by monoclonal antibodies and Western blotting

Monoclonal antibodies raised against single serotype components of a Pseudomonas aeruginosa vaccine have been shown to bind to the O antigen region of lipopolysaccharide (LPS). Outer membrane (OM) proteins, prepared by detergent treatment of envelope fractions and by EDTA/sonication treatment of whole cells, were separated on sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS—PAGE), electrophoretically transferred to nitrocellulose membrane and reacted with LPS-specific monoclonal antibodies. The patterns produced revealed that many of the protein bands were in fact protein—LPS complexes.