Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek’s disease virus (MDV) transformed cell line MSB1 by targeting RORA

Figures

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  Fig. 1. The expression levels of gga-miR-155 in MSB1 cells transfected with (a) gga-miR-155 mimic and (b) gga-miR-155 inhibitor. ** P <0.01, * P <0.05

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  Fig. 2. gga-miR-155 promoted proliferation of MSB1 cells Time-dependent growth curve of MSB1 cells transfected with (a) gga-miR-155 mimic and (b) gga-miR-155 inhibitor and their respective controls. * P <0.05 versus control

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  Fig. 3. gga-miR-155 accelerated progression through the cell cycle. Flow cytometry histograms show the proportion of cells in the different phases of the cell cycle following transfection with (a) gga-miR-155 mimic, (b) gga-miR-155 mimic NC, (d) gga-miR-155 inhibitor and (e) gga-miR-155 inhibitor NC. Bar graphs comparing the percentage of cells in the G1, S and G2 phases of the (c) gga-miR-155 mimic/NC and (d) gga-miR-155 inhibitor/NC transfected groups.* P <0.05

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  Fig. 4. gga-miR-155 blocked apoptosis in MSB1 cells. Flow cytometry dot plots showing the percentage of live and apoptotic cells in the (a) gga-miR-155 mimic, (b) gga-miR-155 mimic NC, (c) gga-miR-155 inhibitor and (d) gga-miR-155 inhibitor NC groups. (e) Percentage of apoptotic cells in the different groups. * P <0.05

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  Fig. 5. Effects of gga-miR-155 on the migrate and invasion of MSB1 cells. Bar graphs showing the migration and invasion rates of MSB1cells transfected with (a) The migration of MSB1 cell treated with gga-miR-155 mimic/NC &inhibitor/NC (b) The invasion of MSB1 cell treated with gga-miR-155 mimic/NC &inhibitor/NC. * P <0.05

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  Fig. 6. gga-miR-155 directly binds to and regulates the expression of RORA gene in MSB1 cells. a The potential gga-miR-155 seed region at the binding site in RORA 3’ UTR (position 516-522) was predicted by TargetScan. b Relative luciferase activity in the different groups. c Expression levels of RORA mRNA in the different groups. d Immunoblot showing expression of RORA protein in the different groups. Error bars indicate the standard deviation from three independent replicates. ** P <0.01, * P <0.05