Identification of Some Trace Lipids in Honey

SUMMARY The trace lipids of cotton honey were extracted with Skellysolve B and diethyl ether. The crude lipid extract was subjected to trans‐esterification, purified on a silicic acid column, and analyzed by gas‐liquid chromatography. For further confirmation the crude lipid extract was saponified and the fatty acids identified by reverse‐phase chromatography. The infrared spectrum of the crude lipid extract and methyl esters showed absorption bands typical of alkane and ester groups. Palmitic and oleic acids were respectively found in relative concentrations of 26.7 and 60.370, along with small amounts of lauric, stearic, and linoleic acids.