What question did this study set out to answer?

To determine whether the lipid second messenger ceramide triggers apoptosis in PC12 cells and to elucidate the underlying protease-dependent mechanism.

January 20, 1997FEBS Letters

Ceramide induces apoptosis in PC12 cells

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To determine whether the lipid second messenger ceramide triggers apoptosis in PC12 cells and to elucidate the underlying protease-dependent mechanism.
Treated PC12 cells with exogenous ceramides (C2-ceramide, C6-ceramide, natural ceramide) and control analogs (C2-dihydroceramide) across various doses, durations, and plating densities.
Assessed apoptosis morphologically via nuclear appearance and internucleosomal DNA fragmentation.
Evaluated the involvement of interleukin-1beta converting enzyme (ICE)-like proteases using the peptide inhibitor Z-VAD.FMK.
Exogenous C2-ceramide, natural ceramide, and C6-ceramide induced apoptosis in a dose- and time-dependent manner, whereas C2-dihydroceramide and related lipids failed to trigger cell death.
The apoptotic potency of ceramide depended critically on cell plating density.
Co-treatment with the ICE-like protease inhibitor Z-VAD.FMK completely prevented ceramide-induced apoptotic nuclear changes.

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Implication

In vitro study demonstrates ceramide-induced apoptosis dependent on caspase-like proteases in PC12 cells, indicating a lipid signaling pathway for programmed cell death.

Cite This Study

Hartfield et al. (1997) studied this question.

synapsesocial.com/papers/6a9124ec5d48fdaeb8cafc3a https://doi.org/10.1016/s0014-5793(96)01460-3

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Implication

In vitro study demonstrates ceramide-induced apoptosis dependent on caspase-like proteases in PC12 cells, indicating a lipid signaling pathway for programmed cell death.

Ceramide induces apoptosis in PC12 cells

Key Points

Discussion

Implication

Cite This Study

Ceramide induces apoptosis in PC12 cells

Key Points

Discussion

Implication

Cite This Study

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