What question did this study set out to answer?

To determine whether reactive nitrogen intermediates, particularly nitric oxide generated through anaerobic respiration, mediate biofilm dispersal in Pseudomonas aeruginosa.

October 18, 2006Journal of BacteriologyOpen Access

Involvement of Nitric Oxide in Biofilm Dispersal of Pseudomonas aeruginosa

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Authors

NBNicolas BarraudInstitut Pasteur DHDaniel J. HassettUniversity of North Carolina at Chapel Hill SHSung-Hei HwangChungnam National University

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Implication

Laboratory study demonstrates that low-dose nitric oxide triggers biofilm dispersal in Pseudomonas aeruginosa, suggesting a potent adjunctive strategy to improve antimicrobial biofilm eradication.

Key Points

To determine whether reactive nitrogen intermediates, particularly nitric oxide generated through anaerobic respiration, mediate biofilm dispersal in Pseudomonas aeruginosa.
Exposed mature P. aeruginosa biofilms to sublethal concentrations of the nitric oxide donor sodium nitroprusside (SNP) ranging from 25 to 500 nM.
Assessed biofilm dispersal phenotypes in targeted genetic deletion mutants defective in nitric oxide production (DeltanirS) and degradation (DeltanorCB).
Evaluated the clearance of established biofilms on glass surfaces following combined treatment with 500 nM SNP and antimicrobial agents (tobramycin, hydrogen peroxide, or sodium dodecyl sulfate).
Sublethal concentrations of SNP (25 to 500 nM) effectively induced the dispersal of sessile biofilm cells into free-swimming planktonic bacteria.
The DeltanirS mutant lacking metabolic NO generation failed to disperse, whereas the NO reductase-deficient DeltanorCB mutant demonstrated greatly enhanced dispersal.
Coadministration of 500 nM SNP markedly improved the efficacy of tobramycin, hydrogen peroxide, and sodium dodecyl sulfate in clearing established P. aeruginosa biofilms.

Cite This Study

Barraud et al. (2006) studied this question.

synapsesocial.com/papers/6a9129d6ca59a4cbdea151df https://doi.org/10.1128/jb.00779-06

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Implication

Laboratory study demonstrates that low-dose nitric oxide triggers biofilm dispersal in Pseudomonas aeruginosa, suggesting a potent adjunctive strategy to improve antimicrobial biofilm eradication.

Involvement of Nitric Oxide in Biofilm Dispersal of Pseudomonas aeruginosa

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Implication

Key Points

Cite This Study

Involvement of Nitric Oxide in Biofilm Dispersal of Pseudomonas aeruginosa

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Member takes

Implication

Key Points

Cite This Study

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